SIRT2 deacetylates α-tubulin K40 (44); and, as expected, treatment of cultured HepG2 hepatocellular carcinoma cells with FLS-359 for 24 hours increased the level of acetylated α-tubulin in HepG2 cells by a factor of about 3 (Supplemental Figure 1A). This evidence concerns the gene SIRT2 and hepatocellular carcinoma.