GLTP and urinary tract infection: In this study, to evaluate the role of glutamate transporter GltS, as well as two other glutamate transporters, GltP and GltI, in E. coli tolerance and pathogenicity, we constructed their deletion mutants in both E. coli K12 strain BW25113 and uropathogenic E. coli UTI89, followed by analysis of their tolerance to various antibiotics and stressors in vitro and their pathogenicity in human bladder epithelial cells and mouse urinary tract infections.