Consequently, Huh 7 cells with forced GFP‐core expression presented with significantly suppressed level of granzyme B and perforin (94% and 94%, respectively) secreted by splenic CD8+ cells induced by anti‐CD3 antibody treatment compared with that of hepatoma cells cotransfected with ISX‐specific shRNAi construct (48% and 47%) or GFP (83% and 84%) alone (Figure 6g,h). The gene discussed is GZMB; the disease is hepatocellular carcinoma.