Confocal microscopy revealed local accumulations of eGFP+ TRP-1 CD4+ T cells in association with MHC-II-expressing CD11c-Venus+ immune cells within tumour invasive margins in HCmel12 CRISPR-ctrl but not in HCmel12 Trp1-KO tumours (Extended Data Fig. 5c–f). The gene discussed is ITGAX; the disease is neoplasm.