CAT and adenoviridae infectious disease: The quantitative reverse transcriptase-PCR (qRT-PCR) procedure showed that gene expressions of the loxP-flanked CAT gene and the Gluc gene were significantly downregulated and upregulated, respectively, in MIN-6 cells after the Cre-adenovirus infection (Supplementary Fig. 3A, B), indicating Cre to mediate recombination of the transgene cassette as intended.