We initially calculated the univariate and multivariate analysis of overall survival (OS) rate, including the expression of genes belonging to the PUS family (PUS1, PUS3, PUS7, PUS10, PUS7L, PUSL1), patients’ age, gender, race and Tumor Node Metastasis (TNM)-stage, and set grade as co-variate (Figure 3A, 3B). The gene discussed is PUSL1; the disease is neoplasm.