Noticeably, overexpression of PUS1 by PUS1-LV decreased E-Cadherin protein expression but increased Vimentin protein levels in A498 cells (Figure 4S), whereas silencing of PUS1 by PUS1-shRNA-LV induced E-Cadherin expression but repressed Vimentin protein levels in ACHN cells contrarily (Figure 4T), suggesting PUS1 expression levels were associated with RCC cell mobility and metastasis. This evidence concerns the gene CDH1 and renal cell carcinoma.