Conversely, infection of PUS1-shRNA-LV resulted in the opposite effects in ACHN cells, including lowered viability at different time points, particularly at 48 h, 72 h and 96 h, down-regulated migration, suppressed invasive capability, and decreased colony numbers (Figure 4F, 4K–4N, 4Q, 4R). The gene discussed is PUS1; the disease is infection.