Of these, 25 potential risk factors were statistically significant for amputation age, sex, diabetes duration, neuropathy, albumin, decreased GFR, being on dialysis, TG level, HTG, hypercholesterolaemia, total cholesterol, HDL-C, hyperlipidaemia, atherosclerotic peripheral arteries, CAD, hypertension, congestive heart failure, CRP, haematocrit, WBC, platelet count, presence of infection, severe bacterial infection, gangrenous Wagner and ulcer pathogenesis (Table 1 and Table 2). The gene discussed is CRP; the disease is hypertensive disorder.