PTEN and hepatocellular carcinoma: Ambiguous results were obtained in other studies, where PTEN downregulation was observed after treatment with 10.0 mmol/L for 24 h in 3T3-L1 preadipocyte cells [67] and with 1.0–10.0 mmol/L for 24–72 h in Huh7.5 hepatocytes [68], whereas upregulation occurred with 27.0 mmol/L after 24 h in hepatocellular carcinoma cell lines MHCC97H and Hep3B [69].