Therefore, we plan to continue our investigation on the antidepressant effects of DIZE by quantifying the levels of Ang (1–7) and alamandine to confirm whether Ang (1–7) or alamandine actually increase in the brain after DIZE administration, and by performing acute and chronic administration of DIZE using animal models of depression such as olfactory bulbectomy, social defeat, or chronic mild stress. This evidence concerns the gene ANGPT1 and major depressive disorder.