The ability of ANGPTL3 to differentiate diseases status from control were evaluate using ROC curve analysis and the result represented a good ability for ANGPTL3 to distinguish between control and T2DM (cut-off: 202.01 pg/mL, area under curve (95% CI): 0.864 (0.800, 0.928), p < 0.001) (Fig. 2a) and DN (cut-off: 201.95 pg/mL, area under curve (95% CI): 0.925 (0.882, 0.968), p < 0.001) (Fig. 2b). Here, ANGPTL3 is linked to type 2 diabetes mellitus.