We thoroughly evaluated six different data analysis pipelines and found the pipeline consisting of an alignment with either NGMLR or Minimap2 and variant calling with BCFtools to perform best with regard to detecting variants in GBA1. With our established and validated workflow, we demonstrated that the frequency of the two common GBA1 risk variants for PD (i.e., p.L483P and p.N409S) in Norwegian patients with PD is 4.3% and higher than in the general population (1.1%). Here, GBA1 is linked to Parkinson disease.