Importantly, the SEA primer set could amplify only SEA trait and Hb Bart’s hydrops fetalis as shown in Fig. 2B. In addition, the THAI primer set could amplify only THAI trait while negative reaction in normal subject, SEA trait and Hb Bart’s hydrops fetalis (--SEA /--SEA) (Fig. 2C). The gene discussed is GSTM1; the disease is hydrops fetalis.