To evaluate extracellular catalase (EC CAT), mice bearing established 4T1 breast tumors and CT26 colorectal carcinoma tumors were treated with 20 μg CAT-ABP + alum or alum alone i.t. on day 7, then 2 days later, tumors were harvested and RNA was extracted for sequencing (Fig. 4A, top). This evidence concerns the gene CAT and colorectal carcinoma.