Six hours post-infection levels of Pyk2 or pPyk2 (Y402) were measured by Western blot in infected cells and controls, including those where PF-431396 hydrate had been added (Fig. 2) PF-431396 hydrate significantly reduced levels of pPyk2 (Y402) in LF82-infected and uninfected RAW 264.7 cells post-treatment (5 and 10 μM; Fig. 2a). The gene discussed is PTK2B; the disease is infection.