To further validate our findings, we quantified the expression levels of MIR99AHG and the crucial genes in the prognostic model (IL1RL1, SPAG16, FAM110B, ANKRD6, ACSS3, CORO2B and TNFAIP8L3) in 15 GC samples and their adjacent nontumor tissues. This evidence concerns the gene IL1RL1 and gastric cancer.