Next, as many reports have suggested a significant association between neuroinflammation and depression,35, 36 and our previous research has implied that PPM1F may regulate the neuroinflammation reactivity,37 therefore, we investigated the neuroinflammation‐related molecules after PPM1F knockdown in the mPFC, we found that the number of iba1+ cells, a marker for microglia was increased in the PPM1F knockdown group (Figure S6A, t(10) = 2.7850, p = 0.0193). This evidence concerns the gene AIF1 and depressive disorder.