The combination of metformin, which upregulated KLF10 expression, and evodiamine, a non-toxic Notch-3 inhibitor, did not only reduced sphere formation activity and stem cell markers expression in Panc-1-pLKO-shKLF10 cells, but also exhibited its therapeutic efficacy in suppressing the tumor growth of the Panc-1shKLF10 cells in orthotopic implant mice. Here, NOTCH3 is linked to neoplasm.