Serum concentrations of MYL3, CA3, FGG and LDHB were elevated in DMD patients compared to age-matched healthy individuals (Fig. 2 plot A, D, G, J and M) and followed decreasing trajectories with increasing age in the patient cohort (Fig. 2 plot C, F, I, L and O) in comparison to the controls (Fig. 2 plot B, E, H, K, N), confirming previous experimental results (Supplementary Fig. 2) and conclusions [29, 37, 38]. This evidence concerns the gene MYL3 and Duchenne muscular dystrophy.