HOXD-AS2 and glioblastoma: Furthermore, we investigated its sub‐cellular localization in U87 and LN229 cell lines using FISH and sub‐cellular fractionation analysis; With reference to the corresponding indicators of GAPDH (cytoplasm), 18S RNA (cytoplasm), and U6 (nucleus), HOXD‐AS2 was predominantly localized in cytoplasm of glioblastoma cells (Figure 3B,C).