Furthermore, we investigated its sub‐cellular localization in U87 and LN229 cell lines using FISH and sub‐cellular fractionation analysis; With reference to the corresponding indicators of GAPDH (cytoplasm), 18S RNA (cytoplasm), and U6 (nucleus), HOXD‐AS2 was predominantly localized in cytoplasm of glioblastoma cells (Figure 3B,C). This evidence concerns the gene GAPDH and glioblastoma.