Neutrophils also accumulate during NASH and contribute to hepatocyte injury40 and we cannot rule out contributions of Irg1+ neutrophils toward lipid metabolism in the liver; however, Irg1 expression by neutrophils is independent of WD feeding, neutrophil cell numbers across all cellular zones of the liver are dwarfed by those of monocytes and macrophages in the steatotic liver17 and we show that itaconate expression is confined to F4/80-sorted macrophages. Here, ACOD1 is linked to Wilson disease.