To better understand the role of Pirin in a melanocytic context, we firstly studied Pirin expression in primary melanocytes and melanoma cell lines by RT-qPCR and in Western Blots, demonstrating that primary melanocytes exhibit generally stronger and more homogenous Pirin expression than melanoma cell lines, which had significantly lower expression and more heterogeneity among the different cell lines analyzed. The gene discussed is PIR; the disease is melanoma.