Based on our findings regarding the DEGs detected following PIR silencing in melanocytes, and the significant increase in E2F1 (p value 5 × 10−4, FDR 0.022) and c-MYC (p value 7.8 × 10–5, FDR 0.006) expression observed, and given that these are target genes of JARID1B30,31, their expression was evaluated in Pirin overexpressing melanoma cells by RT-qPCR. The gene discussed is E2F1; the disease is melanoma.