F5 and hyperinsulinemic hypoglycemia, familial, 4: In practice, these predictions serve two purposes; first, it could anticipate the manifestation of FV-deficiency in patients harboring new single-point, non-synonymous mutations in the F5 gene; and second, by understanding which mutations are more likely to impair FV’s function, researchers can avoid these substitutions when performing targeted mutations aiming to improve FV’s activity, stability and half-life.