To investigate this notion, we constructed macrophage CD146 conditional knockout (M-KO) mice (Fig. S2A, B) and established a subcutaneous transplant tumor mouse model using a mouse liver cancer cell line (Hepa1-6) or colorectal cancer cell line (MC38) in these mice and their control wild-type littermates (M-WT). This evidence concerns the gene MCAM and colorectal cancer.