Conversely, ACR at high doses increased intracellular Ca2+ concentration accompanied by an increase in clCasp3+ cells, which probably contributed to the enhanced transamidase activity of nuclear TG2, cross-linking of nuclear transcription factors such as Sp1, and apoptosis in HCC cells [32, 36, 37]. The gene discussed is TGM2; the disease is hepatocellular carcinoma.