To characterize the malignant cells and their TIME of PCNS DLBCL, we performed 5′ single-cell RNA sequencing (scRNA-seq) on CD45+CD19+ and CD45+CD19− immune cells isolated from a cohort of 8 immunocompetent patients (two replicates for P73; Supplementary Table S1). The gene discussed is CD19; the disease is diffuse large B-cell lymphoma.