α-Synuclein (Snca) was slightly but significantly increased in 3xTg-AD mice exposed to alcohol versus WT controls but not in 3xTg-AD mice or WT mice exposed to alcohol versus WT controls of pyramidal neurons in L5 and L2/3 (L2/3 IT, L5 IT; Extended Data Fig. 4-1). Here, SNCA is linked to Alzheimer disease.