In 3xTg-AD mice exposed to alcohol versus WT controls but not in 3xTg-AD mice or WT mice exposed to alcohol versus WT controls, complement C4b and metallothionein-1 (Mt1) were selectively increased in astrocytes, whereas among IFN-regulated genes, Ifitm3 was upregulated in astrocytes, endothelial cells, and L2/3 IT, and Ifi27 was upregulated in astrocytes and L5 ET (Fig. 4A, Extended Data Fig. 4-1). Here, MT1A is linked to Alzheimer disease.