Furthermore, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis of HCT116 (Fig. 2E) and U2OS (Fig. 2F) cancer cell lines demonstrated p53-dependent CROT mRNA is increased along with the p53 target genes p21 and MDM2 in response to several p53-activating conditions. This evidence concerns the gene TP53 and cancer.