In the current study, through bioinformatic analysis and IHC staining validation, we found that PDGF-BB was overexpressed in CCA, and multiple immunofluorescence staining analyses revealed that PDGF-BB and the CAF marker α-SMA were mostly co-localized, further confirming that PDGF-BB was mainly expressed in CAFs in CCA. This evidence concerns the gene ACTA1 and cholangiocarcinoma.