To reveal the downstream functional signaling pathway of PDGF-BB/PDGFFRβ, we performed western blotting analysis of members of the classical downstream pathways of the PDGF-BB/PDGFFRβ signaling axis, such as AKT, JNK, ERK1/2, YAP, FAK, GSK3β, and P65, in CCA cells after treatment with or without PDGF-BB. The gene discussed is MAPK8; the disease is cholangiocarcinoma.