Through KEGG analysis, we confirmed that the expression of SRPX2 was closely related to the PI3K/AKT signaling pathway, and WB verified that the level of P-PI3K decreased after knocking down SRPX2. Furthermore, the results of thermal cluster analysis and the volcanic map of SRPX2 showed that the expression of PTGDS, GREM1, LAMA4, S100A14, PREX2, and GLS2 in thyroid carcinoma was positively correlated with SRPX2. In addition, the RT-qPCR results showed that their mRNA levels decreased to different degrees after SRPX2 knockdown. The gene discussed is AKT1; the disease is thyroid gland carcinoma.