To further confirm the finding, immunohistochemical detection of PD-L1, CD86, and CD206 in cancer tissues and adjacent tissues of 60 HCC patients was conducted by the research group, and it was found that PD-L1, CD206, and CD86 were significantly overexpressed in cancer tissues, which might be attributed to the suppression of immune response caused by PD-L1 overexpression in the immune microenvironment of HCC (Zhang et al. 2018). This evidence concerns the gene CD274 and cancer.