PFKFB3 (gene that encodes 6‐phosphofructo‐2‐kinase/fructose‐2,6‐biphosphatase 3), a glycolytic activator, is highly expressed in NPC‐derived EVs and facilitates cell growth, migration, and angiogenesis in NPC by influencing the cell cycle and EMT. Here, PFKFB3 is linked to nasopharyngeal carcinoma.