The homeostatic relationship between host and gut microbiota is of significant importance for the host’s health, and the dysbiosis of gut microbiota is associated with many metabolic diseases, such as nonalcoholic fatty liver disease (NAFLD) and obesity.1 Previous studies have highlighted the roles of the liver-, muscle- and adipose tissue-gut microbiota axes in host metabolism.2–4 Notably, all of these tissues are targets of growth hormone (GH), which prompted us to explore whether there is a crosstalk between GH signaling and gut microbiota and the mediators involved in their interaction. Here, GH1 is linked to obesity due to melanocortin 4 receptor deficiency.