Consistent with the bioinformatics analysis results, the immunofluorescence staining results demonstrated marked expression of NCAM1 (a marker of NK CD56 bright cells) and F4/80 (a marker of macrophages) in human HCC tissues with high TMEM147 expression (Figures 6(i) and 6(j)). This evidence concerns the gene TMEM147 and hepatocellular carcinoma.