As shown in CheckMate-227 for advanced non-small-cell lung cancer, dual blockade of PD-1 and CTLA-4 significantly improved OS compared with chemotherapy in both patients with TMB≥10 mut/Mb and those with TMB<10 mut/Mb via F1CDx (Hellmann et al., 2019). The gene discussed is CTLA4; the disease is non-small cell lung carcinoma.