Purinergic receptors express the distinctive feature that on stimulation of many of the P2Y subtypes, they respond by causing a quick change in signals of intracellular [Ca+2] which is useful for the screening of agonist and antagonist of purinergic receptors by performing calcium flux assay in P2Y transfected 1321N1 astrocytoma cell line (Govindan and Taylor, 2012). The gene discussed is P2RY2; the disease is astrocytoma (excluding glioblastoma).