The ROC curve analysis was used to analyze the diagnostic accuracy of the investigated plasma circSERPINA3, miR-944, GPC3, and AFP for HCV-induced HCC group, the AUC values (Table 4) were analyzed to calculate the sensitivity and specificity (Table 4) (See Fig. 6a, 6b, c, d) respectively. This evidence concerns the gene AFP and hepatocellular carcinoma.