According to the MCC algorithm in the cytoHubba plug-in of Cytoscape, the top 10 genes were selected as hub genes including PTGS2、IL1B、IL6、NQO1、ZFP36、SIRT1、ATF3、CDKN1A、EGR1、NOX4. It has been reported that the fibrosis development of NASH experienced five stages, F0 to F4, representing no fibrosis, sinusoidal fibrosis, sinusoidal and portal fibrosis, bridging fibrosis, and cirrhosis respectively (38). This evidence concerns the gene NOX4 and Cirrhosis.