NOX4 and metabolic dysfunction-associated steatohepatitis: According to the MCC algorithm in the cytoHubba plug-in of Cytoscape, the top 10 genes were selected as hub genes including PTGS2、IL1B、IL6、NQO1、ZFP36、SIRT1、ATF3、CDKN1A、EGR1、NOX4. It has been reported that the fibrosis development of NASH experienced five stages, F0 to F4, representing no fibrosis, sinusoidal fibrosis, sinusoidal and portal fibrosis, bridging fibrosis, and cirrhosis respectively (38).