We conjecture there may be the following reasons: 1) The duration of MCD-diet feeding was too short to reach a certain degree of disease severity; 2) The expression level of Sirt1 may be more related to the stage of fibrosis in NASH, but no fibrosis was observed in our models; 3) Differences in gene expression exist between human and mouse. Here, SIRT1 is linked to metabolic dysfunction-associated steatohepatitis.