Loss- and gain-of-function assays to determine comprehensively the biological functions of lncRNA PRRT3-AS1 in NSCLC progression showed that it played a pro-oncogenic role in NSCLC; PRRT3-AS1 knockdown evidently inhibited NSCLC cell growth and motility in vitro, whereas PRRT3-AS1 overexpression exerted the opposite effects. The gene discussed is PRRT3; the disease is non-small cell lung carcinoma.