Similarly, in our study, we found that TLR4 expression was not significantly affected by KGM or 5-FU treatment alone in 5-FU-resistant HCC cells, while the combined treatment of KGM and 5-FU exhibited significant inhibition of TLR4 expression, which was reversed by transfection of TLR4 overexpression vectors. Here, TLR4 is linked to hepatocellular carcinoma.