Tie-2 is a receptor tyrosine kinase, the activated or phosphorylated form of Tie-2 is controlled by tyrosine phosphatase SHP2, however, the relationship and detail mechanisms of SHP-2 in TEMs remain unclear, thereby the conditional-knockout SHP-2 in myeloid or monocytes/macrophages were used in CT26 tumor-planted mouse model and primary F4/80, Tie2 + TEMS were sorted by flow cytometry. Here, PTPN11 is linked to neoplasm.