To observe the expression of hub genes at mRNA level in BRCA patients, we analyzed the mRNA levels of TCGA paired samples, and the results suggested that AZU1 and ENO1 were not significantly different between tumor tissues and normal tissues (Figure 7C, D), while LTF was significantly downregulated and LCP1 was upregulated (Figure 7A, B). The gene discussed is ENO1; the disease is invasive breast carcinoma.