Besides, we found 12 well-known candidate genes for MDD in the PLS1 genes [30], among which 10 genes were significantly associated with regional alterations in MCN, including HTR1A, CNR1, SST, TAC1, PDE1A, MAOA, CUX2, CHRM2, HTR5A, and ARRA2A (PFDR < 0.05; Supplementary Results 9 and Fig. S8). This evidence concerns the gene HTR5A and major depressive disorder.