Therefore, we hypothesized that the restoration of granulosa cell MEG3 expression and potential MEG3 targeting of miR-21 involved in granulosa cell apoptosis are important mechanisms of acupuncture in the treatment of PCOS; neuronal MEG3 in granulosa cells may be an important target involved in acupuncture treatment of PCOS. The gene discussed is MEG3; the disease is polycystic ovary syndrome.