In rats with PCOS, acupuncture inhibited miR-21-3p expression by targeting LncMEG3, while LncMEG3 silencing targeted the activation of miR-21-3p expression in ovarian granulosa cells, promoting FSH and E2 production and inhibiting T, AMH and LH production, thereby inhibiting early and late apoptosis in ovarian granulosa cells and improving abnormal follicle development (Fig. 9). The gene discussed is PLOD1; the disease is polycystic ovary syndrome.