To investigate the specific mechanism by which the MEG3/miR-21-3p axis is involved in the development of follicular abnormalities in PCOS, we used a rat model of PCOS induced by DHEA, along with silent MEG3 lentivirus intervention in PCOS rats (Figs. 2C, 3A, 4A, 5A, 6A). This evidence concerns the gene MEG3 and polycystic ovary syndrome.