Subsequently, we measured the daily change of IFN-γ and TNF-α levels in serum to monitor the kinetic response of systemic inflammatory reaction following Eimeria challenge infection (Figure 5B) since 3-1E is an immunodominant antigen of Eimeria inducing early host inflammatory response (Lillehoj et al., 2000, 2005). This evidence concerns the gene IFNG and infection.