Results from principal component analysis (PCA) of the composition of intestinal microbiota in the CKO and WT mice in a steady state as well as in the DSS-induced colitis showed that Senp2 deficiency in T cells did not alter the general gut microbiome in a steady state (Fig. 4a, b), while samples from DSS-induced WT and CKO mice had distinct clusters, indicating an association of alteration of intestinal microbiota caused by the deletion of Senp2 and more severe inflammation (Fig. 4c, d). This evidence concerns the gene SENP2 and colitis.