The strong enrichment of monocytes in PBMC samples and Kupffer cells in Normal phenotype was consistent with physiological distributions of myeloid cells whereas the pro-fibrogenic macrophage Mp_02_SPP1 in ICC and conventional DC featuring LAMP3 expression (DC_CCL19) exhibited significant concentration in ICC and HCC respectively as frequently discussed in literature26,40,41 (Fig. 8a). The gene discussed is SPP1; the disease is intrahepatic cholangiocarcinoma.