Figure 10A–10C suggested that the protein and mRNA of HOXB9 were upregulated in GBM samples, consistent with IHC results (Figure 10D). Next, we found that HOXB9 expressions were increased in glioma cell lines (SW1783, U87, and SW1088) compared with normal human astrocytes (NHA) (Figure 10E). Therefore, we downregulated the expression of HOXB9 in U87 cells. Western blotting and qPCR results verified the knockdown status of HOXB9 in U87 cell lines (Figure 10F). This evidence concerns the gene HOXB9 and glioma.