,29 In dose-response experiments in AR-negative (PC3, DU145) and AR-positive (LNCaP, 22Rv1) PCa cell lines, PCa cell growth was highly inhibited with toyocamycin with EC50 values ranging from 3.5 to 8.8 nmol/L (Figure 5, A and B). This evidence concerns the gene AR and posterior cortical atrophy.