To further characterize the influence of nuclear cyclin D1 overexpression on NER, we expressed either WT cyclin D1 or the nonphosphorylatable variant cyclin D1 (T286A), both epitope-tagged with Flag-hemagglutinin (Flag-HA), in the human melanoma cell line WM1366, which we previously determined to be S phase NER-proficient (32). The gene discussed is CCND1; the disease is melanoma.