ANXA5 and breast cancer: Nevertheless, a previous study mentioned above found that carotenoid lutein induced minimal apoptotic cell death in breast cancer cells MDA-MB-468 and MCF-7 treated with 2 μM lutein for 24 h, having a 5.21% of early-stage apoptotic (annexin V+/PI−) population in MDA-MB-468 cells and a minor increased (<10%) late-stage apoptotic/necrotic (annexin V+/PI+) cell fraction in MDA-MB-468 cells, but not in MCF-7 cells [49].